Optically Selective Two-Photon Uncaging of Glutamate at 900 nm
نویسندگان
چکیده
منابع مشابه
Optically selective two-photon uncaging of glutamate at 900 nm.
We have synthesized a 7-diethylaminocoumarin (DEAC) derivative that allows wavelength-selective two-photon uncaging at 900 nm versus 720 nm. This new caging chromophore, called DEAC450, has an extended π-electron moiety at the 3-position that shifts the absorption spectrum maximum of DEAC from 375 to 450 nm. Two-photon excitation at 900 nm was more than 60-fold greater than at 720 nm. Two-photo...
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متن کاملChemical Two-Photon Uncaging: Neurotechnique a Novel Approach to Mapping Glutamate Receptors
tons should produce glutamate in proportion to the Functional mapping of neurotransmitter receptors resquare of the light intensity and do so primarily at the quires rapid and localized application of transmitter. focal plane. We tested this strategy in hippocampal pyThe usefulness of caged glutamate for this purpose ramidal neurons by comparing the performance of a has been limited, because ph...
متن کاملChemical Two-Photon Uncaging: a Novel Approach to Mapping Glutamate Receptors
Functional mapping of neurotransmitter receptors requires rapid and localized application of transmitter. The usefulness of caged glutamate for this purpose has been limited, because photolysis by unfocused light above and below the target cell limits depth resolution. This problem is eliminated by using a double-caged glutamate that requires absorption of two photons for conversion to active g...
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ژورنال
عنوان ژورنال: Journal of the American Chemical Society
سال: 2013
ISSN: 0002-7863,1520-5126
DOI: 10.1021/ja4019379